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thp1 dual tlr4 md  (InvivoGen)


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    Structured Review

    InvivoGen thp1 dual tlr4 md
    Thp1 Dual Tlr4 Md, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thp1+dual+md2+cd14+tlr4/THP1-Dual+MD2-CD14-TLR4+Cells/pm41962328-342-0-3
    Average 94 stars, based on 10 article reviews
    thp1 dual tlr4 md - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    other:

    Article Title: Carbohydrate fatty acid monosulphate ester adjuvant enhances the immunogenicity of influenza antigens via TLR4/2-dependent mechanisms.
    Article Snippet: After co-culture, the cells were washed and stimulated with phorbol 12-myristate 13-acetate (50 ng/ml/0.5 x 106 cells) and ionomycin (500 ng/ml/0.5 x 106 cells) (Sigma– Aldrich), along with GolgiStop (BD Biosciences) for 4 hrs.

    Gene Knockout:

    Article Title: Carbohydrate fatty acid monosulphate ester adjuvant enhances the immunogenicity of influenza antigens via TLR4/2-dependent mechanisms
    Article Snippet: After staining the cells for the surface marker CD4, and CD127, fixation/permeabilization was carried out with an intracellular fixation & permeabilization buffer (eBioscience) for intracellular staining of IFN-γ, IL-17A, IL-4, FoxP3 to determine the frequency of Th1, Th2, Th17, and Treg subpopulations by flow cytometry. .. THP1-Dual, THP1-Dual MD2-CD14-TLR4, and THP1-Dual KO-TLR4 reporter cells (Invivogen) were resuspended at a concentration of 100,000 cells per well in a 96-well U-bottom plate in 200 μl RPMI 1640 Medium, GlutaMAX Supplement (Gibco), supplemented with 25mM HEPES, 10% FBS, 100 IU/ml penicillin, and 100 μg/ml streptomycin. ..

    Concentration Assay:

    Article Title: Carbohydrate fatty acid monosulphate ester adjuvant enhances the immunogenicity of influenza antigens via TLR4/2-dependent mechanisms
    Article Snippet: After staining the cells for the surface marker CD4, and CD127, fixation/permeabilization was carried out with an intracellular fixation & permeabilization buffer (eBioscience) for intracellular staining of IFN-γ, IL-17A, IL-4, FoxP3 to determine the frequency of Th1, Th2, Th17, and Treg subpopulations by flow cytometry. .. THP1-Dual, THP1-Dual MD2-CD14-TLR4, and THP1-Dual KO-TLR4 reporter cells (Invivogen) were resuspended at a concentration of 100,000 cells per well in a 96-well U-bottom plate in 200 μl RPMI 1640 Medium, GlutaMAX Supplement (Gibco), supplemented with 25mM HEPES, 10% FBS, 100 IU/ml penicillin, and 100 μg/ml streptomycin. ..



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